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superscript iv one-step reverse transcription-polymerase chain reaction (rt-pcr) kit  (Thermo Fisher)


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    Thermo Fisher superscript iv one-step reverse transcription-polymerase chain reaction (rt-pcr) kit
    Superscript Iv One Step Reverse Transcription Polymerase Chain Reaction (Rt Pcr) Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+one-step+reverse+transcription+polymerase+chain+reaction+(rt-pcr)+kit/superscript+iv+one+step+reverse+transcription+polymerase+chain+reaction++rt+pcr++kit/pm36029850-100-0-11
    Average 90 stars, based on 1 article reviews
    superscript iv one-step reverse transcription-polymerase chain reaction (rt-pcr) kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS
    Article Snippet: .. The isolated RNA was converted into cDNA using cDNA synthesis kit (SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA). ..

    Article Title: Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS.
    Article Snippet: .. The isolated RNA was converted into cDNA using cDNA synthesis kit (SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA). ..

    cDNA Synthesis:

    Article Title: Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS
    Article Snippet: .. The isolated RNA was converted into cDNA using cDNA synthesis kit (SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA). ..

    Article Title: Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS.
    Article Snippet: .. The isolated RNA was converted into cDNA using cDNA synthesis kit (SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA). ..

    Article Title: The Quantitative Trait Loci Mapping of Rice Plant and the Components of Its Extract Confirmed the Anti-Inflammatory and Platelet Aggregation Effects In Vitro and In Vivo
    Article Snippet: The concentration of RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, ND-2000, Waltham, MA, USA). .. The cDNA library was synthesized with the qPCRBio cDNA Synthesis kit (SuperScript IV One-Step RT-PCR System, Thermo Fisher, Cat. 12594025, Waltham, MA, USA) using 3 μL of the RNA sample and 1 μL of the primer (100 pmol). ..

    One Step RT-PCR:

    Article Title: Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS
    Article Snippet: .. The isolated RNA was converted into cDNA using cDNA synthesis kit (SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA). ..

    Article Title: Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS.
    Article Snippet: .. The isolated RNA was converted into cDNA using cDNA synthesis kit (SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA). ..

    Article Title: Mutational and splicing landscape in a cohort of 43,000 patients tested for hereditary cancer
    Article Snippet: .. For RT-PCRseq, total RNA is converted to complementary DNA (cDNA) by reverse transcriptase polymerase chain reaction (RT-PCR) using a one-step approach with custom-designed primers for the target region (Superscript IV one-step RT-PCR kit, Thermo). ..

    Article Title: The Quantitative Trait Loci Mapping of Rice Plant and the Components of Its Extract Confirmed the Anti-Inflammatory and Platelet Aggregation Effects In Vitro and In Vivo
    Article Snippet: The concentration of RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, ND-2000, Waltham, MA, USA). .. The cDNA library was synthesized with the qPCRBio cDNA Synthesis kit (SuperScript IV One-Step RT-PCR System, Thermo Fisher, Cat. 12594025, Waltham, MA, USA) using 3 μL of the RNA sample and 1 μL of the primer (100 pmol). ..

    other:

    Article Title: Three-Generation Study of Male Rats Gestationally Exposed to High Butterfat and Bisphenol A: Impaired Spermatogenesis, Penetrance with Reduced Severity
    Article Snippet: Total RNA was isolated from frozen testes using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA) and reverse transcribed using the Superscript IV One Step RT-PCR kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Article Title: Amentoflavone attenuates oxidative stress and neuroinflammation induced by cerebral ischemia/reperfusion in rats by targeting HMGB1-mediated TLR4/NF-κB signaling pathway
    Article Snippet: The DNA removal, reverse transcription and PCR amplification reactions were carried out using the One-Step SuperScript IV RT-PCR kit (Thermo Fisher Scientific) and specific oligonucleotide primers listed in .

    Sampling:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Polymerase Chain Reaction:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Article Title: Mutational and splicing landscape in a cohort of 43,000 patients tested for hereditary cancer
    Article Snippet: .. For RT-PCRseq, total RNA is converted to complementary DNA (cDNA) by reverse transcriptase polymerase chain reaction (RT-PCR) using a one-step approach with custom-designed primers for the target region (Superscript IV one-step RT-PCR kit, Thermo). ..

    Extraction:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Reverse Transcription:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Article Title: Mutational and splicing landscape in a cohort of 43,000 patients tested for hereditary cancer
    Article Snippet: .. For RT-PCRseq, total RNA is converted to complementary DNA (cDNA) by reverse transcriptase polymerase chain reaction (RT-PCR) using a one-step approach with custom-designed primers for the target region (Superscript IV one-step RT-PCR kit, Thermo). ..

    Concentration Assay:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    RNA Extraction:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Filtration:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    Pore Size:

    Article Title: Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.
    Article Snippet: Nucleospin RNA virus Kit (Macherey-Nagel) PrimeScriptTM One Step RT-PCR Kit; RT-qPCR diagnostic panel assays (US CDC, 2019-nCoV RUO Kit and the positive control 2019-nCoV_N_Positive Control by Integrated DNA Technologies). .. 1.48 × 105–3.90 × 105 Secondary Effluent: 2.51 × 105 Tertiary Effluent: No detection a (La Rosa et al., 2020b); Milan and Rome, Italy Municipal wastewater; Grab sampling; 250 mL; − 20 ◦C Pasteurization (57 ◦C, 30 min) PEG-Dextran precipitation One step PCR Inhibitor removal kit NucliSENS miniMAG semiautomated extraction system (bioMerieux,) RT-PCR SuperScript III/ IV Reverse Transcriptase (ThermoFisher Scientific); a a a (continued on next page) A . Buonerba et al. JournalofHazardousMaterials415(2021)125580 4 Table 1 (continued ) Reference; Location Sample type; Sampling mode; Volume; Storage temperature Sample pretreatment Concentration method Treatment for PCR inhibitors RNA extraction Detection/ Quantization Concentration in genome copies/L Viability; (infectious samples/ total samples) Influent Effluent (Zymo Research) Kit PlatinumTM SuperFiTM Green PCR Master Mix, Thermo), (Rimoldi et al., 2020a,b); Milan, Italy Municipal wastewater; Grab sampling; 500 mL; Specific temperature not reported (samples were under refrigeration) Filtration (0.7 μm and 0.2 μm nominal pore size) Not carried out n.r. .. QIAMP VIRAL RNA mini kit (Qiagen, Hilden, Germany) Real-Time RT-PCR a a Test in Vero E6 cells; Influent: (0/ 8) Effluent: (0/ 4) (Bar Or et al., 2020); Israel Municipal wastewater; Composite sampling (24 h); 0.25–1 L; -20 ◦C or − 80 ◦C Centrifugation a. PEG or alum precipitation, centrifugation, b. Centrifugal filtration (Amicon, MWCO of 30 kDa) n.r.

    cDNA Library Assay:

    Article Title: The Quantitative Trait Loci Mapping of Rice Plant and the Components of Its Extract Confirmed the Anti-Inflammatory and Platelet Aggregation Effects In Vitro and In Vivo
    Article Snippet: The concentration of RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, ND-2000, Waltham, MA, USA). .. The cDNA library was synthesized with the qPCRBio cDNA Synthesis kit (SuperScript IV One-Step RT-PCR System, Thermo Fisher, Cat. 12594025, Waltham, MA, USA) using 3 μL of the RNA sample and 1 μL of the primer (100 pmol). ..

    Synthesized:

    Article Title: The Quantitative Trait Loci Mapping of Rice Plant and the Components of Its Extract Confirmed the Anti-Inflammatory and Platelet Aggregation Effects In Vitro and In Vivo
    Article Snippet: The concentration of RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, ND-2000, Waltham, MA, USA). .. The cDNA library was synthesized with the qPCRBio cDNA Synthesis kit (SuperScript IV One-Step RT-PCR System, Thermo Fisher, Cat. 12594025, Waltham, MA, USA) using 3 μL of the RNA sample and 1 μL of the primer (100 pmol). ..



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    Thermo Fisher superscript iv one-step reverse transcription-polymerase chain reaction (rt-pcr) kit
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